Microbiology Test 1

The proper blood-to-broth ratio for blood cultures to reduce the antibacterial effect of serum in adults is:

The most appropriate method for collecting a urine specimen from a patient with an indwelling catheter is:

Which of the following groups of specimens would be acceptable for anaerobic culture?

Sodium polyanetholsulfonate (SPS) is used as an anticoagulant for blood cultures because it:

The optimal collection of a wound specimen for culture of anaerobic organisms is a:

The most important variable in the recovery of organisms in adult patients with bacteremia (bacterial sepsis) is:

Virus transport medium should contain agents that:

A bronchoscopy sample with the request for culture of Legionella is sent to the laboratory. The correct plating protocol is:

A community hospital microbiology laboratory is processing significant numbers of stool cultures because of an outbreak of diarrhea following heavy rains and flooding in the county. A media that should be incorporated in the plating protocol is:

A male urethral discharge specimen submitted for culture should be inoculated to:

Which selective medium is used for the isolation of gram-positive microorganisms?

Campylobacter jejuni isolation requires the fecal specimen be:

Which of the following specimen requests is acceptable?

A cerebrospinal fluid specimen containing only 2 drops of CSF is collected by a lumbar puncture from a febrile 25-year-old male and is submitted for a stat Gram stain and culture. The direct specimen Gram stain is reported as many neutrophils and no microorganisms seen. The remaining drop of CSF should be inoculated to:

A diabetic foot swab from an 82-year-old woman with recurrent infections is submitted for culture. The Gram stain reveals:

• many neutrophils, no squamous epithelial cells
• many gram-negative bacilli
• many gram-positive cocci in chains

The physician requests that all pathogens be worked up. In addition to the sheep blood, chocolate and MacConkey agar plates routinely used for wound cultures, the technologist might also process a(n):

Which of the following is the most appropriate specimen source and primary media selection?

Which of the following is the most appropriate organism and media combination?

A Gram stain from a swab of a hand wound reveals:

• moderate neutrophils
• no squamous epithelial cells
• moderate gram-positive cocci in clusters
• moderate large gram-negative bacilli

Select the appropriate media that will selectively isolate each organism.

An organism that must be incubated in a microaerophilic environment for optimal recovery is:

Vibrio parahaemolyticus is best isolated from feces on: