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Question 1 of 20
1. Question
Media used to support growth of Legionella pneumophila should contain the additives:
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Question 2 of 20
2. Question
The best medium for culture of Bordetella pertussis is:
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Question 3 of 20
3. Question
A 21-year-old patient presents with pharyngitis. A throat swab is collected and submitted for anaerobic culture. This specimen should be:
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Question 4 of 20
4. Question
An antibiotic used to suppress or kill contaminating fungi in media is:
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Question 5 of 20
5. Question
A sputum specimen is received for culture and Gram stain. The Gram-stained smear from this specimen is seen in the image (total magnification 100x):
The technologist’s best course of action would be to:
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Question 6 of 20
6. Question
A vaginal/rectal swab is collected from a pregnant patient to screen for group B Streptococcus colonization. The best medium to inoculate the specimen to is:
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Question 7 of 20
7. Question
When processing a patient specimen for Gram stain and culture, the proper use of a biological safety cabinet includes:
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Question 8 of 20
8. Question
A wound specimen grows 2 colony types on sheep blood agar and 1 clear colony type on MacConkey agar. Sheep blood agar growth is documented as:
- colony type #1 swarming over entire plate, Gram stain: gram-negative bacilli
- colony type #2 white colony, Gram stain: gram-positive cocci in clusters
The best way to isolate colony type #2 from colony type #1 is to subculture:
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Question 9 of 20
9. Question
A differential medium that can be used as a primary isolation agar producing predictable colored colonies that can be distinguished from other organism colony types describes:
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Question 10 of 20
10. Question
Anticoagulants acceptable for use with blood, bone marrow and synovial fluid specimens that are to be cultured include:
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Question 11 of 20
11. Question
Appropriate culture requirements for a specimen from a patient suspected of having tularemia include:
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Question 12 of 20
12. Question
The primary isolation of Neisseria gonorrhoeae requires:
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Question 13 of 20
13. Question
Acceptable specimen sources for culture of anaerobic bacteria includes:
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Question 14 of 20
14. Question
In general, anaerobic infections differ from aerobic infections in which one of the following?
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Question 15 of 20
15. Question
An expectorated sputum is sent to the laboratory for culture from a patient with respiratory distress. The direct specimen Gram stain shows many squamous epithelial cells (>25/lpf) and rare neutrophils. The microscopic appearance of the organisms present include:
- moderate gram-positive cocci in chains and diplococci
- moderate gram-negative diplococci
- moderate palisading gram-positive bacilli all in moderate amounts
This Gram stain is most indicative of:
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Question 16 of 20
16. Question
Upon review of a sputum Gram stain, the technician notes that all the neutrophil nuclei in the smear stained dark blue. The best explanation for this finding is the:
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Question 17 of 20
17. Question
The image depicts a Gram stain (final magnification 1000x) of a knee fluid from a patient who has recently undergone knee replacement surgery:
The best interpretation of this Gram stain is:
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Question 18 of 20
18. Question
The principle difference between the Ziehl-Neelsen acid-fast stain technique and the Kinyoun acid-fast stain technique is the:
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Question 19 of 20
19. Question
Relapsing fever in humans is caused by:
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Question 20 of 20
20. Question
Three sets of blood cultures are obtained from an adult patient with fever and suspected endocarditis. Growth in one aerobic bottle is seen after 5 days incubation, and the Gram stain is seen in the image. This indicates that:
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